Technical FAQs

      1. Does BOT Bioscience produce antibodies in house?

      Yes. BOT Bioscience Inc. has extensive experience in preparing antibodies by our proprietary platform. We have developed 10,000+ antibodies against a variety of targets including virus, CD molecules, drug targets, and enzymes.

      2. Do you update the information of antibody in data sheet if there are any changes?

      Yes. All information in our data sheet are the latest data for all products.

      3. Can I apply for a free sample？

      Yes, some products can be applied for free samples.

      4. Can I get discount for BOT Bioscience products？

      In general, BOT Bioscience provides uniform discounts for bulk orders and some new products.

      5. What is the difference between clone ID and lot number?

      Clone ID is the internal unique identity number assigned to each mAb clone, while lot number is corresponding to each batch of antibody mass production. The same clone ID can have different lot numbers.

      6. How many kinds of native antibodies exist in vivo?

      Five isotypes, , including IgA, IgD, IgE, IgG and IgM.

      7. How to determine the affinity of the product?

      Several methods have been employed for calculating the Ka, e.g. SPR and BLI.

      8. Can cells be stained for FCW and analyzed the following day?

      In general, stained cells can be analyzed the following day if they are fixed and stored appropriately. But analysis occurs on the same day for best results.

      9. Is there epitope information for your antibodies?

      We does not determine the epitope for most of its antibodies.

      10. What is the amino acid sequence of your antibody?

      Our antibody sequence is confidential and cannot be provided, but we can provide information about the immunogen of the antibody.

      11. Can you provide the optimal working concentration of the antibody?

      The product specific pages and datasheets provide a recommended concentration for each validated application.

      12. Can I use your antibodies for a matched pair ELISA with a standard from another company?

      Yes, but protein folding or sequence differences in the antibody-binding region can lead to poor (or no) recognition of the standard.

      13. Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

      Antibody products cannot be used for treatment, only for scientific research.

      14. Do you guarantee a minimum titer?

      For rabbits, we guarantee a minimum titer of 10,000 in at least one animal for all products. For monoclonal antibodies, the titer of different antibodies is also different.

      15. Does BOT-Bioscience provide antibodies with multiple applications?

      BOT-Bioscience guarantees its antibodies work for the applications and react with the species that listed on the website.

      16. Which buffer is used to store antibodies?

      We use 0.01M PBS (pH 7.4).

      17. How are affinity purified antibodies produced?

      To produce anti-rabbit IgG affinity purified secondary antibodies, anti-rabbit IgG is passed by a column containing immobilized rabbit IgG.

      18. How do I reconstitute the antibody?

      We recommend you briefly centrifuge the vial to ensure all of the antibody is at the bottom of the tube.

      19. What are the advantages of polyclonal antibodies?

      Polyclonal antibodies can recognize a mixture of different epitopes of the antigen.

      20. How to choose a peptide for immunization?

      Usually the synthesized peptide should have similar structure:
      Peptides derived from the C terminal with N terminal modified by acetylation.
      Peptides derived from the N terminal with C terminal modified by amidation.
      Peptides derived from an internal sequence with both ends modified.